Table 1 Annotated functions of the genetic changes in E. coli C41(DE3) and C43(DE3).
Gene | Function | Mutation | Gene position | Region | Effect |
|---|---|---|---|---|---|
Present only in C41(DE3) | |||||
 proY | Predicted cryptic proline transporter | T → A | 438 | Coding | Synonymous |
 melB | Melibiose:sodium symporter | C → T | 653 | Coding | Ala → Val |
 ycgO | Potassium/proton antiporter | C → A | 1103 | Coding | Gly → Val |
 yhhA | Hypothetical protein | C → T | 290 | Coding | Pro → Leu |
Present only in C43(DE3) | |||||
 dcuS | Sensor of fumarate two-component system; frame-shifted in BL21(DE3) | GCGCC deletion | 866–870 | Coding | Frameshift |
 fur | Ferric uptake regulator | GTC insertion | 392 | Coding | Val insertion |
 lacI | lac repressor | G → T | 574 | Coding | Val → Phe |
 lon | DNA-binding ATP-dependent protease La | IS4 excision | −156 from lon | Intergenic | Activation of Lon protease |
 yibJ | Predicted Rhs-family protein | C → A | 90 | Coding | Synonymous |
 yjcO | Hypothetical protein | A → G | 665 | Coding | Glu → Gly |
 cydA | Cytochrome d terminal oxidase, subunit I | IS1 insertion | −262 from cydA | Promoter | N.D. |
 ccmF ~ ompC* | Listed in Supplementary Table 1 | IS1 deletion | 21-kb large deletion | N.D. |  |
 yjiV ~ yjjN* | Listed in Supplementary Table 2 | IS1 deletion | 37-kb large deletion | N.D. |  |
Present in both C41(DE3) and C43(DE3) | |||||
 l | T7 DNA-directed RNA polymerase | AA −> GT, G −> A | PlacUV5 −10 PlacUV5 CAP binding site | Promoter |  |
 rbsD | Predicted cytoplasmic sugar-binding protein; disrupted by insertion of IS in BL21(DE3) | IS3 excision | 217–1586 | Coding | N.D. |
 yehU | Predicted sensory kinase in two-component system | T −> G | 679 | Coding | Phe → Val |
