Table 2 Sequence differences in the promoter region and coding sequence of T7 RNAP.

From: Comparative genomics and experimental evolution of Escherichia coli BL21(DE3) strains reveal the landscape of toxicity escape from membrane protein overproduction

Strain/Group

Promoter sequence of T7 RNAP

Coding sequence of T7 RNAP

No. of colonies

CAP binding site

−10 to +1 region

BL21(DE3)

PlacUV5

PlacUV5

—

—

C41(DE3) C43(DE3)

PlacUV5

Plac

—

—

BL1†

PlacUV5

Plac

N.D.

3

BL2†

Plac

Plac

N.D.

9

BL3†

PlacUV5

PlacUV5

changed

3

  1. BL, large colonies obtained from BL21(DE3).
  2. †Evolved mutants obtained in this study.
  3. N.D., not determined.
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