Table 3 Sequence differences in the lacI gene.

From: Comparative genomics and experimental evolution of Escherichia coli BL21(DE3) strains reveal the landscape of toxicity escape from membrane protein overproduction

Strain/Group

Genomic location

DE3 phage-inserted

lac operon

C43(DE3)

G574T (V192F)

No mutation

CL1†

C583A (R197S)

No mutation

CL2†

No mutation

G223A (A75T)

CL3†

G673A (G225S)

G223A (A75T)

  1. CL, large colonies obtained from C41(DE3).
  2. †Evolved mutants obtained in this study.
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