Table 1 Primers for the Quantitative RT-PCR.

From: Wnt/β-catenin coupled with HIF-1α/VEGF signaling pathways involved in galangin neurovascular unit protection from focal cerebral ischemia

Gene

Sequences (5′ to 3′)

PCR product (bp)

HIF-1α for

GATGAATCAAAAGCAGTGACGAAGG

106

HIF-1α rev

ATGCCTTAGCAGTGGTCATTTCTTG

VEGF for

CCGTCCTGTGTGCCCCTAATG

101

VEGF rev

CGCATGATCTGCATAGTGACGTTG

Claudin-5 for

GCTTGTGGCACTCTTTGTTACCTTG

193

Claudin-5 rev

AGACACCGGCACCGTTGGATC

Ctnnb1 for

GGACTCTAGTGCAGCTTCTGGGTTC

191

Ctnnb1 rev

ACAGATGGCAGGCTCGGTAATG

Lrp6 for

TGGATGGAACAGAACGGGAGGT

104

Lrp6 rev

GTCGGAGTCAGCCCAGAAGAGC

Tjp1 for

AGCCAGTTCCGCCTCTGTCCA

189

Tjp1 rev

GGGGATAGAAGGTCTGAGGAGGGTC

Fzd1 for

GCTCAATAACGTGGATGCTCTGC

154

Fzd1 rev

TTTCCAGTTTCTCTGTCTTGGT

Factor VIII for

AGTGCCAAGGAGCCATTCGC

185

Factor VIII rev

ATCAGGCTTCCCGTGGAGTTTC

GAPDH for

TGGAGTCTACTGGCGTCTT

138

GAPDH rev

TGTCATATTTCTCGTGGTTCA

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