Figure 5
From: Targeting HER-3 to elicit antitumor helper T cells against head and neck squamous cell carcinoma
Recognition of a naturally processed exogenous antigen by HER-3-reactive CD4 T cells.
The ability of HER-3-reactive CD4 T cells to recognize naturally processed exogenous HER-3 was assessed judging by IFN-γ production. Dendritic cells (DCs) were used as antigen-presenting cells (APCs) and HLA-DR-matched or -unmatched tumor cell lysates served as sources of HER-3 protein. L243 cells were used to verify the HLA-DR restriction of these responses. DC without antigen or pulsed with PBMC lysate was used as a negative control and DC pulsed with HER-3872−886 was used as a positive control. Columns: means of triplicate measurements, bars: SEM. The results shown are representative of 3 separate experiments.
