Figure 6

pan HER-family inhibitor augments antitumor responses of HER-3-reactive CD4 T cells.

(A,B) HLA-DR expression on the surface of SAS, HPC92Y, HSC4, Sa-3 and HSC3 cells was evaluated using flow cytometry after 48-h preincubation with IFN-γ (100 IU/ml) alone or with dacomitinib (1 μM) by flowcytometry. Columns: means of triplicate measurements, bars: SEM. The results shown are representative of 3 separate experiments. (C) We used an IFN-γ assay to evaluate the responses of HER-3-reactive T cells (O3 and n24) to tumor cells pretreated for 48 h with IFN-γ (100 IU/ml) alone or with dacomitinib (1 μM): n.s.: not significant; *p < 0.05. Columns: means of triplicate measurements, bars: SEM. The dead cells and the doublets were eliminated by FSC/SSC gating before the analysis. The results shown are representative of 3 separate experiments.