Figure 4
From: Determination of RNA polymerase binding surfaces of transcription factors by NMR spectroscopy
Competition of RNAP and NusG-CTD for NusE binding.
(a) Displacement of RNAP from NusB:NusEΔ by NusG-CTD. 1D [1H,15N]-HSQC spectra of free NusB:[15N]-NusEΔ, black, NusB:[15N]-NusEΔ in the presence of RNAP in equimolar concentration, light blue and NusB:[15N]-NusEΔ in the presence of RNAP and NusG-CTD (molar ratio 1:1:1, dark blue; 1:1:3, green; 1:1:10, red). (b) Displacement of NusB:NusEΔ from NusG-CTD by RNAP. 2D [1H,15N]-HSQC spectra of [15N]-NusG-CTD, black, [15N]-NusG-CTD in the presence of NusB:NusEΔ in equimolar concentration, green and [15N]-NusG-CTD in the presence of NusB:NusEΔ and RNAP (molar ratio 1:1:1, blue; 1:1:3, red). (c) Detail of the rectangular region in (b). Black arrows indicate the chemical shift changes that occur upon addition of NusB:NusEΔ to [15N]-NusG-CTD, red arrows show the changes upon subsequent addition of RNAP. (d) Schematic representation of the potential functions of a direct NusE:RNAP interaction. Color code as in Fig. 1.
