Figure 4 | Scientific Reports

Figure 4

From: Ion exchanger in the brain: Quantitative analysis of perineuronally fixed anionic binding sites suggests diffusion barriers with ion sorting properties

Figure 4

Prussian blue reaction (PBR) on brain slices loaded with 12.5 mM FeCl3.

(A) untreated; (B; CHASE) pre-treated with chondroitinase ABC; and (C; HASE) pre-treated hyaluronidase. PNs are distinct (asterisks) in the untreated section (A), while treatment with either chondroitinase (B) or hyaluronidase (C) prior to Fe3+-loading abolished PN labeling. Scale bar: 20 μm, applies to all.

Back to article page