Figure 1
From: Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System
Optimization of PFF transfection.
(a) The pEGFP-N1 expression plasmid was transfected into PFFs using the indicated methods. GFP fluorescence was analyzed via fluorescence microscopy and FACS at 24 h post-transfection. (b) Percent of GFP-expressing cells and mean fluorescence intensity of GFP-expressing cells.n = 3. Graphs show the mean ± S.E.M.
