Figure 3
From: Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System
Generation of gene-targeted piglets via SCNT.
(a) Genotypes of a portion of the MSTN mutant single-cell colonies. The target sites are labeled in red and PAM in blue. C8,C10,C14 and C18 are Landrace PFFs while E4,E8,E15,E17,E21,E23 and E35 are Erhualian PFFs. (b) Newborn piglets harboring MSTN mutations and some of the piglets show visible intermuscular boundaries (upper panel) and enlarged tongues (lower panel) compared with the WT controls.
