Figure 4
From: Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System
Identification of MSTN biallelic mutant piglets.
(a) Representative images of H&E-stained sections to compare the total longissimus dorsi muscle cross-sectional area at a × 4 magnification and general morphology at a × 20 magnification. (b) Quantification of number of myofiber nuclei in longissimus dorsi muscle slices of two mutant and two WT piglets. n = 5. Each bar indicates the mean ± S.E.M. (c) Semi-quantitative RT-PCR analysis of MSTN and myogenic regulatory factors in mutant and WT pigs. (d) Western blotting analysis of MSTN levels in mutant and WT piglets.
