Figure 5
From: Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System
Detection of sgRNA#1-mediated off-target effects in mutant pigs.
(a) PCR products covering potential off-target site 16 were subjected to T7 endonuclease I (T7E1) assays. Two pigs (asterisk) showed cleavage bands indicating non-specific mutations in OTS-16. (b) Further cloning of the PCR products confirmed the mutations. The target sites and PAM of OTS-16 are labeled in red and blue, respectively.
