Table 1 Comparison of the mutation efficiency induced by sgRNA alone or sgRNA + HDR template.
From: Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System
Cell | Targeting vector | HDR template | Mutation | TA colonies | Mutation efficiency | HDR efficiency (%) |
|---|---|---|---|---|---|---|
Erhualian PFFs | sgRNA#1 | – | 17 | 78 | 21.79% | – |
Erhualian PFFs | sgRNA#2 | – | 10 | 80 | 12.50% | – |
Erhualian PFFs | sgRNA#1 | ssDNA-85 | 19 | 55 | 34.55% | 2/55(3.64%) |
