Table 2 SCNT results for the generation of gene-targeted pigs.
From: Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System
Target gene | Cell pool | Transferred embryos | No. recipients | No. (%) pregnancies | No. born | No. (%) mutants |
|---|---|---|---|---|---|---|
MSTN | C10,C14,C16 | 195 | 1 | 1 | 2 | 1 |
C8,C10,C14,C16 | 320 | 2 | 2 | 7 | 6 | |
C8, C10, C16 | 440 | 2 | 1 | 3 | 1 | |
Total | 955 | 5 | 4 (80%) | 12 | 8 (67%) |
