Figure 3 | Scientific Reports

Figure 3

From: Identification of key amino acid residues responsible for internal and external pH sensitivity of Orai1/STIM1 channels

Figure 3

Effects of extracellular protons on Orai2/STIM1 and Orai3/STIM1.

(A,B) Representative recordings at pHo 5.5, 7.4 and 9.5. (C) Concentration-dependent effects of external protons on Orai2/STIM1 and Orai3/STIM1 in comparison with Orai1/STIM1. Current amplitude was normalized to that at pHo 7.4. (D) Dose-response curve obtained by normalizing to maximal current amplitude. Best-fit of the dose-response curves yielded pKa of 8.32 ± 0.14 for Orai2/STIM1 and 8.52 ± 0.21 for Orai3/STIM1, identical to the pKa obtained in (C). Note that the dashed lines in (C,D) represent the data of Orai1/STIM1 taken from Fig. 1C,D.

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