Figure 5
Results of cell proliferation assays after the hDPSCs were exposed to materials derived from the 3 aging cycles.
(A) MTT assay. Mitochondrial dehydrogenase activities of hDPSCs exposed to different materials are expressed as percentages relative to the unexposed hDPSCs negative control (100%). (B) Cellular DNA content. Statistical analyses were only conducted for hDPSCs exposed to the two hydraulic cements and unexposed hDPSCs (negative control) from the 3 aging periods. For the factor “material” in each chart, groups labeled with the same designators (numerals for 1st cycle, upper case letters for 2nd cycle and lower case letters for 3rd cycle) are not significantly different (P > 0.05). For the factor “aging cycle” in each chart, cycles from the same hydraulic cement that are connected with a horizontal bar are not significantly different (P > 0.05). For unexposed hDPSCs, there are no differences in activities among the 3 cycles (P > 0.05; horizontal bar not shown).
