Table 1 Reaction conditions and quantitation of target compounds in synthetic schreibersite reaction mixtures.
From: Nucleoside phosphorylation by the mineral schreibersite
Reaction ID (Ad = adenosine, U = uridine) | Initial Reaction Conditions | Phosphorylated nucleotide concentration in μM (Yield based on total P) | Final Reaction Conditions | |||||
|---|---|---|---|---|---|---|---|---|
Initial pH | Time (days) | Additional Reagents | 5′ | 3′ | 2′ | Final pH | Total P (mM) | |
Ad1 | 6.5 | 14 | NDa | ND | ND | 6.5 | 0.24 | |
Ad2 | 6.5 | 14 | Urea | 73 (2.9%) | <5b | <5b | 9.5 | 2.5 |
Ad3 | 6.5 | 14 | MgSO4, Urea | 210 (4.2%) | 41 (0.8%) | 38 (0.8%) | 9 | 5 |
Ad4 | 12.5 | 2 | K2CO3 | 360 (0.8%) | 130 (0.3%) | 94 (0.2%) | 10.5 | 47 |
Ad5 | 11.5 | 2 | NH4OH | 250 (5.7%) | 22 (0.5%) | 29 (0.5%) | 9.5 | 4.4 |
U1 | 6.5 | 14 | ND | ND | 6.5 | 0.65 | ||
U2 | 6.5 | 14 | Urea | 56 (0.6%) | 65 (0.7%) | 9.5 | 10 | |
U3 | 6.5 | 14 | MgSO4, Urea | 54 (1.5%) | 84 (2.4%) | 8 | 3.5 | |
U4 | N/Ac | 14 | Fe2NiP (1.5 g), Urea | 200d (0.5%) | ~330d (0.8%) | N/A | 43 | |
