Figure 1 | Scientific Reports

Figure 1

From: Live detection and purification of cells based on the expression of a histone chaperone, HIRA, using a binding peptide

Figure 1

Purification and confirmation of specificity of TM2.

(a) The RP-HPLC profile of the synthesized peptide. TM2 was eluted at 15.5 minutes. (b) The western blot analysis for testing the specificity of the peptide-targeting using biotin-tagged peptide in oral cancer cell lines. The right panel shows the western blot using HIRA antibody. (c) Immunofluorescence detection of HIRA in fixed HSC-4 cells using TM2-FITC or HIRA antibody. (d) Immunofluorescence of fixed ES cells using TM2-FITC. Hoechst 33342 dye was used to visualize the nucleus. The scale bar represents 10 μm.

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