Figure 4
DE induced by 6.25 ng/ml Activin and DMSO further differentiated into immature gut epithelium.
The ability of DE to differentiate into immature gut epithelium, triggered by a low dose of Activin and DMSO, was characterized. (A) The scheme for hiPS cell differentiation. After DE differentiation with 6.25 ng/ml Activin and DMSO, further incubation with BIO and DAPT induced differentiation into immature gut epithelium. (B–E) BIO and DAPT induced intestinal epithelium. (B) At day 6, SOX17+CDX2+ cells appeared. (C–E) At day 8, CDX2+ cells began to express VILLIN (C) and LGR5 (D,E), as shown by immunocytochemistry (C,D) and flow cytometry (E). Caco2 cells are used as a positive control for VILLIN expression. Scale bar; 50 μm.
