Figure 4
Effect of gefitinib on BIM and mTOR expression.
(a). PC-9 and H3255 cells were treated with DMSO vehicle and 5 nM or 50 nM of gefitinib for 24 hours. Lysates were prepared and run on gels for western blot with specific antibodies. Actin was used as the loading control. Incubation of cells with gefitinib induced a dose-dependent increase of BIM but did not change mTOR expression. (b) PC-9 cells were treated with indicated concentrations of gefitinib for 5 days. BIM and MTOR mRNA levels were assessed by qRT-PCR. Incubation of PC-9 cells with gefitinib induced a dose-and time-dependent increase of BIM but did not affect MTOR mRNA expression. (c) 11–18. H1975 and H1650 cells were treated with DMSO vehicle and 5 nM or 50 nM of gefitinib for 24 hours. Gefitinib did not induce BIM or inhibit mTOR expression in the gefitinib-sensitive 11–18 and the gefitinib-resistant H1975 and H1650 cells.
