Figure 4
TGFβR1 is a direct target of miR-181b.
(a) Venn diagrams showed the number of genes identified as potential targets of miR-181b using three predicting search programs of TargenSCan, miRanda and miRBD. (b) Relative luciferase activity was analysed after wild-type or mutant 3′-UTR reporter plasmids were co-transfected with pGLE/miR-181b or miR-181b-NC in H1299 cells. (c) qRT-PCR and western blot detection of TGFβR1 mRNA and protein expression in A549 or A549/DDP cells transfected with miR-181b mimics or anti-miR-181b. U6 or GAPDH was used as an internal control. *P < 0.05; **P < 0.01.
