Figure 3 | Scientific Reports

Figure 3

From: Characterization of Pseudooxynicotine Amine Oxidase of Pseudomonas putida S16 that Is Crucial for Nicotine Degradation

Figure 3

SDS-PAGE analysis of site-directed mutagenesis proteins and FAD content detection of Pnao.

(A) SDS-PAGE analysis of purified wildtype Pnao, PnaoE131G, PnaoP149S, PnaoE159G, PnaoE162G, PnaoE174G and PnaoP180S. M, marker protein; 1, wildtype Pnao; 2, purified PnaoE131G; 3, purified PnaoP149S; 4, purified PnaoE159G; 5, purified PnaoE162G; 6 purified PnaoE174G; and 7, purified PnaoP180S. (B) FAD content of wildtype, mutants and Pnao with different metal salts. C, control group; W, tungsten; Mo, molybdenum; Fe, ferric iron.

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