Figure 1 | Scientific Reports

Figure 1

From: Busulfan and cyclosphamide induce liver inflammation through NLRP3 activation in mice after hematopoietic stem cell transplantation

Figure 1

Liver pathological changes and numbers of mononuclear cells in liver post HSCT.

At indicated time points after HSCT, mice were sacrificed and liver was isolated and fixed with formaldehyde, dehydrated, waxed and sliced into 4 μm thickness by RM2126 microtome for H&E staining (A). For immunohistochemical staining, the slices were incubated with 3% H2O2 at room temperature and blocked with 5–10% goat serum. Then the slices were incubated with pan-endothelial cell monoclonal antibody (MECA-32) at 37 °C for 1–2 h followed by the incubation with secondary antibody. Color was developed with 3,3′-diaminobenzidine. Mason staining was performed using a commercial kit. A representative staining graph selected from 6 independent stainings in each group was shown. Meanwhile, numbers of infiltrated mononuclear cells in liver were also counted (B).

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