Figure 1 | Scientific Reports

Figure 1

From: TATA boxes in gene transcription and poly (A) tails in mRNA stability: New perspective on the effects of berberine

Figure 1

Characteristics of BBR’s affinities for DNA and RNA sequences.

(a): Controls for buffer, BBR, RNA and DNA. (b): TATA box, GC box and CAAT box. (c): Different TA compositions. (d): Different TATA boxes. In (a–d), the DNA oligos were applied at the same concentration (25 μM). (e): Poly (A). (f): Different concentration of BBR with poly (A) (20 μM). (g): Comparison of poly (A) of deoxyadenine (poly dA) (10 μM) and poly (A) of adenine (poly A) (10 μM). (h): Comparison of poly dA as a single strand (10 μM, s-30 nt) and a double strand (5 μM, d-30 bp). In (a–h), BBR was applied at the same concentration (10 μM). (i): Sequences containing TATA boxes used in (d).

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