Figure 1

Physiochemical changes after exposure to PEG-induced dehydration in callus cultures.
The SCCs were maintained at 25 ± 2 °C, at 16 h photoperiod and treated with varying concentrations of PEG. Quantitative determination of (A) callus fresh weight, (B) cell viability, (C) RWC and (D) electrolyte leakage was carried out with increased PEG concentration. Comparative estimation of (E) fresh weight, (F) cell viability, (G) RWC, (H) electrolyte leakage, (I) MDA and (J) proline content at 10% PEG. The experiments were accomplished in triplicates (n = 3) and average mean values were plotted. Vertical bars in the graphs indicate the S.E.