Figure 2 | Scientific Reports

Figure 2

From: Secretome analysis of chickpea reveals dynamic extracellular remodeling and identifies a Bet v1-like protein, CaRRP1 that participates in stress response

Figure 2

Purity evaluation of chickpea secreted fraction.

(A) Determination of catalase-specific activity in cell lysate and secreted fractions. The SCCs were collected after centrifugation at 3,000 g for 10 min at 4 °C and cell lysates were used as positive control. (B) Immunoblot analysis of secreted proteins with anti-RbcL antibody. An aliquot of 50 μg protein was separated by 12.5% SDS-PAGE and electroblotted onto nitrocellulose membrane. Rubisco was detected using alkaline phosphatase conjugated secondary antibody. Vertical bars in the graphs indicate the S.E.

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