Figure 5

Subcellular localization and secretion analysis of leaderless protein CaRRP1.
Localization was carried out in tobacco leaves using Agrobacterium-mediated transient expression without (A,B) and with (C) BFA (10 μg/mL), an inhibitor of secretion. Yellow fluorescence, visible light and merged images were taken from the epidermal cells, infiltrated with Agrobacterium suspension harboring the constructs encoding CaRRP1-YFP. Bars = 20 μm.