Table 1 Summary of the off-target analysis to isogenic clones.

From: TALENs-mediated gene disruption of FLT3 in leukemia cells: Using genome-editing approach for exploring the molecular basis of gene abnormality

Clone analyzed

Putative off-target sites examined

Mutated sites detected (reference sequence)

Comparison to parental K562 sequence

Clone kw1

20

2

Identical (20/20)

Clone k20

20

2

Identical (20/20)

Clone k114

20

2

Identical (20/20)

Clone k324

20

2

Identical (20/20)

  1. The 20 most possible off-target sites identified using the TALE-NT 2.0 Paired Target Finder were sequenced and aligned with either reference sequence (human reference genome, NCBI Build 36.1) or parental K562 sequence.
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