Figure 3
From: Novel kinase fusion transcripts found in endometrial cancer
Validation of targetable fusions.
(a) Flowchart for discovering targetable fusion transcripts. (b) Identification of CPQ-PRKDC fusion transcripts in JHUEM3 using RT-PCR. Marker represents a 100 bp DNA ladder. ACTB was used as the internal control for RT-PCR. (c) Schematics of the CPQ-PRKDC fusion transcripts. Regions corresponding to CPQ or PRKDC are shown in red or blue, respectively. (d) Sanger sequencing chromatogram showing the reading frame at the breakpoint and putative translation of the fusion protein in the fusion-positive cell line.
