Figure 4

ER expression analysis by Real-time RT-PCR.
Figure 4 shows analysis of mRNA levels of ESR in all cells examined by Real-time RT-PCR. We observed higher level of ESR in MCF-7 and a moderate and/or low expression in the other cell lines. Each sample was normalized to human GAPDH. The bars indicate the mean values of three independent experiments (the bars indicate the s.d.; **p-value < 0.005; *p-value < 0.05; Student’s t-test).