Figure 2
The Chi overexpression induces Notch signaling activation mainly inside clones.
(a-a’) The overexpression of chi generates the boundary of high and low Chi protein (red) regions. Clones were marked with GFP (green) in all the IF results. (b-c”) Immunostaining of Cut (b-b”; red) and Wg (c-c”; red) in the discs of chi overexpression. Cut and Wg activation did not occur at the clones (arrows) near A/P boundary. (b’)and (c’)are the enlarged pictures of (b,c). (d) Quantitative analysis of Cut activated cells around the clones. Shown are the Means ± s.d. (e) A model for the role of Chi on Notch signaling. Notch signaling could be activated along the boundary of different chi expression regions. Around 70% of activated cells are cells with higher Chi protein level. (f-f”) Immunostaining of NICD (red) in the discs of chi26 discs. Clones are marked with dashed line. (g-h”) Immunostaining of Cut (g; red) and Wg (h; red) in the discs of chi RNAi and UAS-NICD overexpression. Cut and Wg are activated in the clones (green). Chi proteins (blue) are totally lost in the clones.
