Figure 3

Representative ATR-responsive genes with variations in DNA methylation and gene expression.

Validation of gene expresison by semi-quantitative RT-PCR (A) and by qRT-PCR (B). (C) RNA-Seq (≥2-fold change, p < 0.05). Data were presented as the ratio of Log₂ [values (μ_{atrazine-treated} /μ_control)]. (D) methylated levels determined by BS-Seq (≥2-fold change). Four loci are LOC_Os06g37300 (CYP701A8), LOC_Os10g38470 (glutathione S-transferase gene, GST), LOC_Os03g28940 (OsJAZ6) and LOC_Os07g48870 (MYB transcription factor). Semi-quantitative RT-PCR and qRT-PCR validation of gene expression in rice exposed to ATR at 0 and 0.4 mg L⁻¹ for 2, 4 and 6 d. The treated samples were pooled and analyzed. Rice ubiquitin was used as an internal reference gene for normalization. Experiments were repeated in triplicate (p < 0.05, ANOVA).