Figure 5

Ectopic expression of TRIM25 reversed the effects of TGF-β inhibitor on the migration and invasion of MKN-28 cells.
(A) MKN-28 cells was transfected with control plasmid (NC) or expression plasmid encoding TRIM25. 48 h after transfection, expression of TRIM25 was analyzed by qRT-PCR and immunoblotting. (B,C) MKN-28 cells transfected with TRIM25 or NC and migration and invasion assay were performed in a transwell chamber containing either DMSO or 10 μM TGF-β inhibitor, SB431542 (Sigma). Scale bar: 100 μm.