Figure 3

Acetic acid and proteins produced by yeast interact to modulate female reproductive behaviours.

(a) Mean number of copulations of one Canton-S male and female exposed to the indicated odorants (concentration of 1% v/v) in presence or absence of Yeast amino acids (Yaa). Number of replicates ranges from 10–29. Error bars indicate S.E.M. (b) Mean number of copulations (b₁) and progeny (b₂) of one wild-type male and female exposed to a protein source with or without Acetic acid (1% v/v). Yeast amino acids “Yaa”, Peptone and Tryptone are at a concentration of 30 g/L. The effect of Acetic acid within one amino acid source condition was tested using GLM, whose resulting p values are reported above the bar graphs: n.s. (non significant); *p < 0.05; **p < 0.001; ***p < 0.0001. Number of pairs tested for each condition is indicated above each bar graph. (c) Mean number of copulations (c₁) and progeny (c₂) of one wild-type male and one female of the indicated genotype exposed to Peptone [30 g/L] with or without the addition of Acetic acid (1%v/v). The effect of Acetic acid within one genotype was tested using mixed effect models, resulting p values are reported above the graphs. See Table S1 for full statistics.