Table 1 Primers used in this study.
Experiments | Genes | GenBank Accession Numbers | Primers | |
|---|---|---|---|---|
Cloning | 1st round PCR | Nested PCR | ||
AmTRPR | KT232312 | F1: GTGTGGAAAGTGATACGTTC | F2: GATGCAGACCGTAGAAGTT | |
R1: CAAGTATGTACTCGTTGCTG | R2: GTCAAGACACGTGACCCG | |||
VdNTL-R | KT232310 | F1: CAGGTTTCCACAGAGCGTC | F2: CACAAGGGCCAAGGTTAAC | |
R1: CTGTTCCACTTTAGCCTACC | R2: GCTTTATTTGCCTAGAGCAC | |||
VdTRP-R | KT232311 | F1: GAGCTTATTAAACTGGCTCG | F2: GCCTCATAATGGATGTCCTC | |
R1: GCCACGAACTAATCAGAAAC | R2: GCTACAGTGTAGTCTAACCCTTC | |||
Tissue-specific RT-PCR | VdNTL | F: ATACCTTTACGATGATTTGCTG | R: CTCCGCCCTTTTTACCAC | |
VdTRP | F: AGGCAAGAAAGCCGCACC | R: CGCATTCCGAAGAACGAT | ||
VdRPS3 | F: GGGCGTAGAGGTGCGCAACGG | R: CGACACCTTCTCGGCGAACAG |
