Figure 1 | Scientific Reports

Figure 1

From: Direct flow cytometry measurements reveal a fine-tuning of symbiotic cell dynamics according to the host developmental needs in aphid symbiosis

Figure 1

Detection of endosymbionts by flow cytometry analysis.

(a–d) Gating strategy used to analyze B. aphidicola cells. (a) Fluorescence histogram overlay of unstained and SYTO9-stained samples, with the dotted line referring to the autofluorescence limit. SYTO9 positive events were selected and debris excluded from the analysis. (b) SYTO9 positive events were viewed in a FSC-W/FSC-H plot to select cells homogeneous in size (gate P1) and depict cell doublets/aggregates. (c) Events in gate P1 were viewed in a SSC-W/SSC-H plot to select cells homogeneous in granularity (gate P2). (d) Cells identified as B. aphidicola were represented on density plot (FSC-A/SSC-A), the color gradient indicating increasing density of dots. (e) Microscopy image of SYTO9-stained symbionts. Scale bars = 5 μm.

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