Figure 5
From: Selection preserves Ubiquitin Specific Protease 4 alternative exon skipping in therian mammals
Exon skipping does not occur in the USP4 gene of the chicken or the zebrafish.
(A) Schematic of the RT-PCR strategy using primers specific for exons 6 and 8. (B) RT-PCR analysis of RNA from the endogenous USP4 gene in chicken. Lane labels specify addition (+) or omission (−) of RT (control) to chicken embryo fibroblast (CEF) RNA extracts. When RT was included a single PCR product of predicted size (329 bp) for the exon-retained transcript was detected. (C) RT-PCR analysis of CEF transfected with the human USP4 minigene construct. Lane labels specify transfection of pDG467 (+) or an irrelevant control plasmid (−) in CEF cells. Both the exon-retained and exon-included products were detected (as in human cells). (D) RT-PCR analysis of RNA from the endogenous USP4 gene in the zebrafish. Lane labels specify addition (+) or omission (−) of template (lane 2) or RT (lane 3) to larval zebrafish RNA extracts. A single amplification product was detected of the size predicted for the exon-retained cDNA (313 bp).
