Figure 5: FITC fluorescence changes of UCU.

(A) Interaction of UCU (in the absence or presence of CAT) with enzymosomal (ESU or ESUC) membranes in buffer-B; (B) Interaction of UCU with enzymosome membranes in buffer-D. (C) Fluorescence change of UCU (in the absence or presence of CAT) in buffer-B induced by heat treatment; (D) Fluorescence change of ESU (or ESUC) in buffer-B induced by heat treatment. (E) Fluorescence change of UCU (in the absence or presence of CAT) in buffer-D induced by heat treatment; (F) Fluorescence change of ESU (or ESUC) in buffer-D induced by heat treatment. The UCU or CAT concentration was fixed at 100 μg/mL. The FITC concentration was fixed at 0.51 μg/mL. UCU + CAT: mixture of UCU and catalase; Blk-ESU (or ESUC): blank ESU or blank ESUC, the blank ESU was exactly the same as blank ESUC, they were both blank enzymosomes; Blk-ESU (or ESUC) + UCU: mixture of blank enzymosome with free UCU; Blk-ESU (or ESUC) + UCU + CAT: mixture of blank enzymosome with free UCU and CAT;