Figure 7: Circular dichroism and gel electrophoresis of free UCU, ESU, UC and ESUC.

(A) Circular dichroism curves. (B,C) Changes of the circular dichroism millidegree at the wavelength of 208 nm and 222 nm. (D) Polyacrylamide gel electrophoresis. (E) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Free UC: mixture of free UCU and CAT. BSA: bovine serum albumin; OVA: ovalbumin. The molecular weights of BSA and OVA were 66 kD and 45 kD, respectively.