Figure 2
From: Nifuroxazide exerts potent anti-tumor and anti-metastasis activity in melanoma
Nifuroxazide induced apoptosis of A375 and B16-F10 cells.
(a) A375 and B16-F10 cells were treated with indicated concentrations of nifuroxazide for 48 h, respectively and then were analyzed by FCM after PI-staining. Quantified values of the apoptosis were shown on the right. Each point represents the mean ± SD for at least 3 independent experiments (*P < 0.05; **P < 0.01; ***P < 0.001 vs vehicle control). The apoptosis of A375 (b) and B16-F10 (c) was analyzed using the Annexin V-FITC/PI dual-labeling technique. The proportions of apoptotic cells, including the early apoptotic cells and the late apoptotic cells, were both shown on the right. (d) Western blot was used to detect the levels of the typical apoptosis-related proteins cleaved caspase-3 and Bcl-2 family proteins. After treated with nifuroxazide, the expressions of cleaved caspase-3, Bcl-2 and Bax were determined by western blot with the expression of β-actin as the internal control. (e) Protein expression was quantified and normalized against β-actin expression. (f) The percentage of Bax/Bcl-2 ratio was presented in the bar graphs.
