Figure 4
From: Nifuroxazide exerts potent anti-tumor and anti-metastasis activity in melanoma
Nifuroxazide inhibits melanoma cell A375 and B16-F10 migration and invasion.
(a,c) Nifuroxazide inhibited the migration of melanoma cells in wound healing assay and transwell assay. In the wound healing assay, cells were seeded in 6 well culture plates and the cell monolayer was wounded manually. Then, the images were taken after treated with nifuroxazide for 24 h. In transwell assay, cells were seeded in the top chamber of transwell with serum-free medium and treated with various concentrations of nifuroxazide. After 24 h, the migrated cells were fixed, stained and quantified. (b) Nifuroxazide inhibited the invasion of melanoma cells in transwell assay. 1 × 105 cells were planted in which were pre-treated with Matrigel on the upper chamber membrane and treated with indicated concentrations of nifuroxazide and the bottom chamber was filled with the complete medium containing 10% FBS. (d) Western blot analysis of A375 cells following nifuroxazide treatment, including the expressions of Stat3/p-Stat3, FAK/P-FAK, Src/P-Src, MMP2, MMP9 and Vimentin.
