Figure 5
From: Nifuroxazide exerts potent anti-tumor and anti-metastasis activity in melanoma
Effects of nifuroxazide on tumor growth in vivo.
Mice implanted with A375 xenografts were treated with 25 and 50 mg/kg/day when the tumors grew to about 100 mm3. (a) Tumor volumes were measured every 3 days and presented as mean ± SD (n = 6, *P < 0.05; **P < 0.01). (b) The bar charts of tumor weight. (c) Tumor cell proliferation was evaluated through immunohistochemical analysis staining with Ki67 and the statistical data of Ki67 positive cell number were shown on the right. (d) The apoptosis of tumor was determined by cleaved caspase-3 immunohistochemical staining and the statistical data of CC-3 positive cell number were shown on the right. Similarly, the immunohistochemical analysis was performed to measure the expressions of MMP-2 (e), MMP-9 (f) and p-Stat3 (g) in tumor tissues. P values for comparison of two groups were determined by 2-tailed Student’s t test (*P < 0.05; **P < 0.01; ***P < 0.001 vs vehicle control).
