Figure 6 | Scientific Reports

Figure 6

From: The Quorum Sensing Inhibitor Hamamelitannin Increases Antibiotic Susceptibility of Staphylococcus aureus Biofilms by Affecting Peptidoglycan Biosynthesis and eDNA Release

Figure 6

HAM alters the amount of eDNA in the biofilm and thereby affects biofilm susceptibility.

(a) The amount of eDNA (average ± s.d.) present in S. aureus Mu50 biofilms formed in the absence (CTRL) or presence of HAM. (b) The amount of eDNA (average ± s.d.) in 24 h old biofilms receiving no treatment (CTRL) or a treatment with HAM or VAN alone or a combination of VAN and HAM (COMB). (c) Cell viability as quantified by CTB staining for S. aureus Mu50 biofilms receiving no pre-treatment, or a pre-treatment with DNase followed by (i) no treatment (CTRL), (ii) a treatment with VAN or (iii) a combination of VAN and HAM (COMB). CTB signals are presented as percentages (average ± s.d.) compared to the signal of an untreated biofilm. (d) The amount of eDNA (average ± s.d.) present in biofilms of S. aureus ATCC 49230, ΔtraP mutant and the traP complemented strain ΔtraP pLI50-U1 traP formed in the absence (CTRL) or presence of HAM. *indicates significant (p < 0.05) differences between the amount of eDNA present in the treated biofilms compared to the CTRL biofilm (a,b,d) or in % signal compared to the biofilms treated with VAN (c). **significant (p < 0.05) differences in percentage signal are observed between biofilm cells treated with a combination of VAN and HAM following no pre-treatment or a pre-treatment with DNase.

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