Figure 2
From: Negative regulation of initial steps in skeletal myogenesis by mTOR and other kinases
High content siRNA screen identifies a subset of kinases that block myogenin induction in myoblasts.
(A) Chart showing experimental conditions and controls used in the screen and quantitation of hits under different conditions. (B,C) Charts showing total number of cells per well (B) or percent of cells expressing Myogenin (C) when cultivated in the indicated medium with or without PD 0332991 (PD) or or with siRNAs targeting individual kinases. (D) Chart showing average total cell number in experimental wells targeting each kinase. Gray and black bars signifying kinases scoring as negative or positive, respectively, for Myogenin induction. (E) Venn diagram showing the positively scoring “hits” with and without PD. PD, PD 0332991; GM, growth media; DM + I, differentiation media plus insulin; *p < 0.05; error bars represent standard deviation.
