Figure 5
From: Negative regulation of initial steps in skeletal myogenesis by mTOR and other kinases
Decreased mRNA and protein/phosphoprotein expression of certain hit kinases correlates with muscle differentiation.
(A) mRNA levels of a subset of the hits in growth medium (GM) versus differentiation medium (DM + I), determined by RNA-Seq. (B) Relative expression of protein (B) and specific phospho-amino acids (C) in a subset of the hits in GM versus DM + I, relative to HSC70 as a loading control, determined using KAM-880 protein microarray. Bars represent standard error for duplicate measurements. Phosphosite activity is listed as stimulatory ( + ) or inhibitory (−) of kinase activity when phosphorylated. Asterisks (*) indicate that an antibody has known or potential cross reactivity with corresponding phosphosite(s) on closely related protein(s). Note: In this figure, data are shown as relative to GM (values in GM are set as 1). The absolute expression of mRNA (A), expression of protein (B) and phospho-amino acid (C) relative to Hsc70 are shown in Supplementary Fig. S5.
