Figure 6
From: Negative regulation of initial steps in skeletal myogenesis by mTOR and other kinases
Mtor knockdown increases the expression of Myogenin and other muscle-specific differentiation genes in myoblasts.
Charts showing mRNA or protein expression of indicated genes in (A,B) C2C12 cells and (C) primary mouse myoblasts cultivated in growth medium (GM) or differentiation medium (DM + I) with control (CTL) siRNA, Mtor knockdown, as indicated. In all cases, mRNA was quantified by qRT-PCR, normalized to Gapdh and shown relative to expression in GM. Of note, Myh7 was excluded from the primary myoblast studies (C) because it was not induced by DM + I. *p < 0.05 compared to GM; error bars represent standard deviation.
