Figure 7
From: Negative regulation of initial steps in skeletal myogenesis by mTOR and other kinases
mTORC1 inhibition by rapamycin or Rptor knockdown increases muscle gene expression, independently from cell cycle arrest.
(A,B) Quantification of (A) mature mRNA expression and (B) G1 cell cycle fraction in C2C12 myoblasts cultivated in growth medium (GM), differentiation medium with insulin (DM + I), or GM with rapamycin for 48 hours at the indicated doses (nM). (C) Aggregate quantification of muscle-specific signature genes (left) or indicated genes (right) in myoblasts cultivated as above with Mtor or Rptor siRNA knockdown. In all cases, mRNA quantified by qRT-PCR, normalized to Gapdh and shown relative to expression in GM. CTL, scrambled siRNA control; *p < 0.05 compared to GM; error bars represent standard deviation.
