Figure 1
From: WISP1 mediates IL-6-dependent proliferation in primary human lung fibroblasts
The WISP1 promoter region contains potential binding sites for transcription factors activated by profibrotic cytokines.
(A) In silico analysis of the WISP1 promoter (2.5 kb upstream of the WISP1 transcription start site (TSS)) revealed potential binding sites for SMAD, TCF, LEF and NF-κB. (B) A reporter construct containing the WISP1 2.5 kb promoter region was transfected into primary human lung fibroblasts (phLFs). phLFs were treated with 100 ng/ml Wnt3a, 2 ng/ml TGFβ1 or 10 ng/ml TNFα for 24 hours followed by measurement of luciferase activity. Wnt3a, TGFβ1 and TNFα all significantly induced luciferase activity as compared to unstimulated conditions (n = 4, *p < 0.05, 1-way ANOVA followed by Neuman-Keuls multiple comparison test).
