Figure 5
From: WISP1 mediates IL-6-dependent proliferation in primary human lung fibroblasts
Loss of WISP1 decreases proliferation of phLFs.
(A) The phLFs showed a dose-dependent increase in proliferation with different concentrations of IL-6 as measured by WST-1. (B–F) phLFs transfected with control (siCtrl) or WISP1-targeting (siWISP1) siRNA had decreased proliferation. (B) Representative bright field images of siCtrl and siWISP1 transfected cells (magnification: 100×). (C) Representative Western Blot of cyclin D1 levels in phLFs after 24 and 48 hours of treatment with siWISP1 and (D) immunofluorescence staining of PCNA (green) and DAPI (blue; magnification: 100×) shows qualitative decreases in cell number and PCNA staining. Decreased proliferation in siWISP1 conditions was measured by (E) WST-1 assay and (F) cell count. (G) Additionally, phLFs were treated with a neutralizing αWISP1 antibody and decreased proliferation was observed by WST-1 assay (n = 3–7; *p < 0.05; **p < 0.01; A: 1-way ANOVA followed by Neuman-Keuls multiple comparison test; E–G: Student’s T-test).
