Figure 2
From: CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation
Validation of the effects of pX330-Satb2 plasmids on pCAG-EGxxFP-Satb2 target plasmid in the mouse brain.
(a) Experimental procedure. Cortical neurons were co-transfected with pCAG-mCherry, pX330-Satb2-272 plus either pCAG-EGxxFP-Satb2-272 or pCAG-EGxxFP-Satb2–524 using in utero electroporation at E15.5 and coronal sections were prepared at P2. The white square indicates the region which was magnified and shown in (b). (b) High magnification confocal microscopic images. Note that EGFP signal was observed in mCherry-positive neurons transfected with pX330-Satb2-272 and pCAG-EGxxFP-Satb2-272, which contained appropriate target sequences (lower panels). In contrast, when pCAG-EGxxFP-Satb2-524, which contained different target sequences, was used as a reporter plasmid, EGFP signals were not observed (upper panels). Scale bar = 20 μm.
